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Automotive engines produce pollutant species which has the potential to damage human health as well as the environment. The toxicity potential of these species depends on the concentration, route, and exposure time. Toxicity studies are required in the current scenario due to increased pollution levels by vehicles used for transportation. This study is a review focused on the toxicity analysis of particulate, elemental (particle associated as soot), and organic carbon (organic fraction, PAHs) emission from the internal combustion engine with conventional and alternative fuels like biodiesel and alcohol. The study is focused on the formation, characterization, and quantification of particulate matter, elemental and organic carbon, and their effect on human health. The other part of the study is focused on mutagenicity (mutation in DNA) and cytotoxicity (cell toxicity) of the particulate emitted from the engines.

Emission regulations mandate the measurement of solid particles of size greater than 23 nm according to UNECE informal working group particle measurement protocol (PMP). The volatile particles from the engine exhaust are removed by heating and dilution in a device according to the UNECE PMP program. The analysis of solid particles from diesel engines requires aerosol conditioning systems, which can effectively remove volatile particles/species with minimum solid particle losses. Currently, the regulations only allow using an evaporation tube for the measurement of solid particles. Different laboratories have also demonstrated other alternatives such as thermodenuder and catalytic stripper for measuring the solid ultrafine particles emitted from the diesel engine. This paper reviews the recent literature related to the thermodenuder and catalytic striper’s design and operating characteristics.

The influence of engine load and fuel premixing ratio (PMR) on unregulated emission from a methanol-diesel dual-fuel RCCI (MD-RCCI) engine is examined in this study. The study focuses on assessing the adverse effects of unregulated emissions (saturated HC, unsaturated HC, carbonyl compounds, aromatic hydrocarbon, NH3, and SO2) on the health of human beings and the environment. To quantify the effect on the environment, the greenhouse gas potential (GWPs), Eutrophication potential (EP), Acidification potential (AP), and Ozone forming potential (OFP) are calculated and presented. The cancer risk potential (CRP) of the carbonyl compounds (HCHO and CH3CHO) is calculated and presented to see the effect on human health. The results demonstrate that at lower engine load, with an increase in PMR, the OFP and CRP for MD-RCCI operation increase significantly, whereas AP, EP, and GWPs decrease. Additionally, with a rise in the load at a constant PMR, the AP, EP and OFP decrease significantly.