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Recent developments in measurement techniques enabled researchers to measure ultra-fine particulates of nano-scale range and provided more evidence that the smaller particulates typically emitted from gasoline engines may have more severe impacts on human respiratory system than the bigger particulates from diesel engines. The knowledge of the characteristics of particulates from gasoline engines, especially, the effect of fuel borne additives is sparse. This work presents the findings from a study into the effect of aftermarket additives on nano-scale particulates. Four commercially available fuel borne additives used in gasoline engines mainly by private vehicle owners in the United Kingdom were selected for this study. The combustion and emission performance of the additive fuels were compared against that of commercially available gasoline fuel using a 4-stroke, throttle body injected gasoline engine.