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The transportation sector adds to the greenhouse gas emissions worldwide. One way to decrease this impact from transportation is by using renewable fuels. Ethanol is a readily available blend component which can be produced from bio blend­stock, currently used blended with gasoline from low to high concentrations. This study focuses on a high octane (RON=97) gasoline blended with 0, 20, and 50, volume % of ethanol, respectively. The high ethanol blended gasoline was used in a light duty engine originally designed for diesel combustion. Due to the high octane rating and high ignition resistance of the fuel it required high intake temperatures of 443 K and higher to achieve stable combustion in in homogeneously charged compression ignition (HCCI) combustion operation at low load. To enable combustion with lower intake temperatures more commonly used in commercial vehicles, ozone was injected with the intake air as an ignition improver.