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A 500 hours endurance test was performed with a heavy-duty engine (Euro IV); MAN type D 0836 LFL 51 equipped with a PM-Kat®. As fuel 100% biodiesel was used that met the European specification EN 14214. The 500 hours endurance test included both the European stationary and transient cycle (ESC and ETC) as well as longer stationary phases. During the test, regulated emissions (carbon monoxide, nitrogen oxides, hydrocarbons and particulate matter), the particle number distribution and the aldehydes emission were continuously measured. For comparison, tests with fossil diesel fuel were performed before and after the endurance test. During the endurance test, the engine was failure-free for 500 hours with the biogenic fuel. There were almost no differences in specific fuel consumption during the test, but the average exhaust gas temperature increased by about 15°C over the time. Emissions changed only slightly during the test.