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Due to increasingly strict emission regulations for IC engines, there is a significant motivation to investigate the relevant physical processes with the objective to reduce the reduction of exhaust gas emissions. Spray characteristics play a progressively important role in the consequent processes of mixture formation, ignition, combustion and pollutant formation in direct injection diesel engines. It is also important to develop an understanding of the atomization qualities of alternative fuels such as Biodiesel fuels as potential substitutions for conventional diesel fuel. In this research, the effect of injection and ambient parameters on spray breakup and atomization of different alternative fuels are investigated using CFD simulation. An Eulerian-Lagrangian approach is implemented in order to study the interaction of the continuous and discrete phases.