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Understanding the causal loop from injection to combustion in modern direct injection engines is essential to improve combustion and reduce emissions. In this work, the section from injection to fuel-evaporation in this causal loop was investigated using different optical measurement techniques, with a focus on drop size measurements using Phase Doppler Anemometry (PDA). One spray jet of a modern DISI multi-hole injector was investigated using gasoline RON 95 fuel and two single component alkane fuels (n-hexane / n-decane). In a first step the macroscopic spray formation and propagation of this spray jet were studied using a 2D-Mie-scattering technique in an optical injection chamber at homogenous charge DISI conditions. Furthermore, the droplet size distribution and mean diameter were determined spatially and temporally resolved for an ambient pressure of 0.3MPa and different ambient temperature (323K / 423K / 523K) conditions in the optical chamber using Phase Doppler Anemometry.