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The objective of this experimental investigation was to analyze the effect of various exhaust gas aftertreatment technologies on particulate number emissions (PN) of an MPFI EU5 motorcycle. Specifically, three different aftertreatment strategies were compared, including a three-way-catalyst (TWC) with LS structure as the baseline, a hybrid catalyst with a wire mesh filter, and an optimized gasoline particulate filter (GPF) with three-way catalytic coating. Experimental investigations using the standard test cycle WMTC performed on a two-wheeler chassis dynamometer, while the inhouse particulate sampling system was utilized to gather information about size-dependent filtering efficiency, storage, and combustion of nanoparticles. The particulate sampling and measuring system consist of three condensation particle counters (CPCs) calibrated to three different size classes (SPN4, SPN10, SPN23).