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The present consumption rates and heavy dependence on fossil fuels pose a humongous threat to the environment. The increased pollution in urban areas is already causing serious sociological, ecological and economic implications. The issue of energy security led governments and researchers to look for alternate means of renewable and environment friendly fuels. Biodiesel has been one of the promising, and economically viable alternatives. The biodiesels are reported to cause reduction in CO, HC and PM emissions. However, NOx emissions are increased in case of biodiesel in CI engine. Therefore, a Urea-SCR over Fe-ZSM5 honeycomb substrate (400cpsi) zeolite catalyst after treatment system is an effective technology to reduce emissions for biodiesel applications. Exhaust gases pass through the catalyst and reactions take place along its surface, consequently converting NOx into nitrogen and H2O.