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This paper is the second of a two part study which investigates the use of advanced combustion modes as a means of improving the efficiency and environmental impact of conventional light-duty vehicles. This second study focuses on drive cycle simulations and Life Cycle Assessment (LCA) for vehicles equipped with Octane-on-Demand combustion. Methanol is utilized as the high octane fuel, while three alternative petroleum-derived fuels with Research octane numbers (RONs) ranging from 61 to 90 are examined as candidates for the lower octane fuel. The experimental engine calibration maps developed in the previous study are first provided as inputs to a drive cycle simulation tool. This is used to quantify the total fuel consumption, octane requirement and tank-to-wheel CO2 emissions for a light-duty vehicle equipped with two alternative powertrain configurations. The properties of the lower octane fuel are shown to affect the vehicle fuel consumption and CO2 emissions significantly.