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In vehicle driving environment, the driver is subjected to the vibrations in horizontal, vertical, and fore-aft directions. The human body is very much sensitive to whole body vibration and this vibration transmission to the body depends upon various factors including road irregularities, vehicle suspension, vehicle dynamics, tires, seat design and the human body's properties. The seat design plays a vital role in the vibration isolation as it is directly in contact with human body. Vibration isolation properties of a seat depend upon its dynamic parameters which include spring stiffness and damping of seat suspension and cushion. In this paper, an optimization-based method is used to determine the optimal seat dynamic parameters for seat suspension, and cushion based on minimizing occupant's body fatigue (occupant body absorbed power). A 14-degree of freedom (DOF) multibody biodynamic human model in 2D is selected from literature to assess three types of seat arrangements.