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When assessing particulate emissions, diesel engine exhausts are usually diluted to suit the design limitations of the measurement devices. Particle number concentrations (PNC) are known to be sensitive to dilution conditions and must be considered when evaluating results. Laboratories employ various experimental techniques to dilute exhaust samples before measurements. The majority of measurement systems use air as dilution a gas, some employ filtered exhaust gas in a closed loop, while others employ nitrogen, where prevention of oxidation reaction is required. In this work, the effect of using air and nitrogen as dilution gases on the PNCs from diesel engine exhausts has been investigated. Our approach explored the use of carbon dioxide (CO2) concentration ratios in diluted and raw exhaust samples, evaluated by non-dispersive infrared (NDIR) analysers to determine dilution conditions of the measured sample.