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The current work investigates the in-cylinder mixing of a fluorescent tracer species inducted into the engine through a small-diameter tube mounted along the inner port wall and the remaining inlet stream in a small-bore utility engine. Planar laser-induced fluorescence (PLIF) measurements were acquired on a single plane, parallel to and approximately 4 mm below the cylinder head deck, throughout the intake and compression strokes. The data were analyzed to qualitatively and quantitatively describe the evolution of the mixture stratification. The highest degree of stratification in the mean field was observed at a timing of 90 crank angle (CA) degrees after top dead center (aTDC) of the intake stroke, which corresponds closely to the point of maximum intake valve lift (105 CA degrees aTDC).