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Diesel engine can be run with biodiesel which has the potential to supplement the receding supply of crude oil. As biodiesel possess similar physiochemical properties to diesel, most diesel engines can run with biodiesel with minimum modifications. However, the viscosity of biodiesel is higher, and the calorific value is lower than diesel. Therefore, when biodiesel is used in diesel engines, it is usually blended with diesel at different proportions. Use of 100% biodiesel in diesel engines shows inferior performance of having lower power and torque. Improving in-cylinder airflow characteristic to break down higher viscous biodiesel and to improve air-fuel mixing are the aims of this research. Therefore, guide vanes in the intake runner were used in this research to improve the performance of diesel engine run with biodiesel.