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Alternative diesel fuels from renewable sources (biodiesels) have increased significantly interest due to their potential CO₂ emission benefits, capability to reduce unburned hydrocarbons and particulate matter emissions, biodegradability and non-toxicity. Biodiesels undergo ageing effects due to autoxidation processes of their molecular chains. Ageing leads to a variety of decomposition products like peroxides, alcohols, aldehydes and carboxylic acids. They are detectable as alterations of chemical properties, odor and taste (rancidity). The characteristics of Rapeseed Methylester (RME), RME aged and diesel sprays have been analyzed for different injection strategies in engines. The tests have been performed on a Bosch second generation common rail solenoid-driven fuel injection system capable of 160 MPa maximum injection pressure, fitted on EURO5 diesel engine for passenger car applications.