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Finite Element simulation of a lower extremity model is used to (1) determine which of the muscle parameters maximum force capacity (Fmax), initial activation levels (Na) and maximum muscle contraction velocity (Vmax) affect ligament strains the most and (2) to identify which muscles affect the knee response the most in low speed, just below the knee, lateral impact. Simulations have been performed with Fmax, Na and Vmax varying from their reference values. Sensitivity of ligament strains to variation in muscle parameters has been studied. It is observed that knee response is more sensitive to Fmax and Na than Vmax. Amongst the muscles varied, reduction in the Fmax and the Na in the hamstring and the gastrocnemius muscles affects the knee ligament strains the most. The hamstring parameters significantly affects the ACL, the PCL as well as the MCL strains whereas, change in the gastrocnemius parameters affects only the MCL strain.