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Accumulation and re-breathing of CO2 in expired air has been investigated as a possible indication for crew discomfort onboard the International Space Station (ISS) Service Module crew quarters. In addition, inadequate airflow contributes to increasing temperature that also leads to crew discomfort. The objective of this study is to evaluate possible medical hazards that can occur when a crewmember is sleeping in the crew quarter of the ISS Service without proper ventilation. This paper investigates a projected increase in CO2 in the enclosure under a no ventilation scenario. A Computational Fluid Dynamics (CFD) model of the crew quarters and of a human body are built to investigate the ventilation profile and the CO2 concentration inside the volume of the crew quarters. Respiratory characteristics of a typical 180-pound crewmember are simulated. The results for the distribution and concentration of expired air at different time intervals and at different locations are presented.