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The NASA Langley Research Center (LaRC), in conjunction with the NASA Goddard Space Flight Center (GSFC), evaluated the impact of an animal science experiment on the Space Station environmental control and life support system (ECLSS). The LaRC-developed ECLSS computer-aided analysis capabilities were used for the evaluation. Equivalent crew size parameters were developed for an animal colony consisting of 96 rodents and 8 squirrel monkeys selected by NASA GSFC as a representative complement for life science research on board the NASA Space Station. Thirteen ECLSS options, along with the analysis assumptions, were established for reclaiming metabolic oxygen (O2) and waste water. In addition, both dedicated and shared options included facilities that were common to both the animal and the crew. The ECLSS options were compared against the Space Station ECLSS baseline provided at the interface requirement review (IRR) at the NASA Johnson Space Center (JSC) in 1986.