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In-cylinder measurements of turbulent integral length scales, carried out during the last 60 degrees of the compression stroke at 600 and 1,000 rpm by a two-probe volume LDV system, were used to assess the capability of the k-ε model used in KIVA-II code. The objective of the paper is to address the following question: what is the most reasonable definition of turbulent length scale in the k-ε model for engine applications? The answer derived from the comparison between KIVA predictions and experiments that showed a fair agreement between the computed turbulent length scale and the measured lateral integral length scale. The agreement is a result of proper choice of the initial swirl ratio and turbulent kinetic energy at inlet valve closure (IVC) by taking into account the LDV measurements and the value of the constant Cμε in the k-ε model equations that relates the turbulent length scale to k and ε.