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The monitoring of spacecraft life support systems for the presence of health threatening microorganisms is paramount for crew well being and successful completion of missions. The union of the molecular biology techniques of DNA probe hybridization and polymerase chain reaction (PCR) offers a powerful method for the detection, identification, and quantification of microorganisms and viruses. This report is an evaluation of the state of PCR science as it applies to the needs of NASA to develop a microbiology monitor for use aboard spacecraft, and a set of recommendations as to the design of a PCR-based microbial monitor for recycled water aboard the ISS.