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Technical Paper

Disinfection Susceptibility of Waterborne Pseudomonads and Legionellae Under Simulated Space Vehicle Conditions

1991-07-01
911402
Experiments were done to examine the sensitivity of various waterborne bacteria from iodinated systems to iodine, and their subsequent recovery and growth, because this halogen is used as a disinfectant in potable water systems on US manned space vehicles. A Pseudomonas aeruginosa isolated from a commercial iodine product was least sensitive when grown in reagent-grade water or phosphate buffered water (PBW) and most sensitive when cultivated on mineral salts medium supplemented with low levels of glucose and glutamate or Brain Heart Infusion (BHI) broth. However, a P. cepacia strain was most sensitive when grown on BHI broth. Isolates from an iodinated potable water system were less sensitive to iodine than Ps-4 while a clinical isolate exhibited intermediate sensitivity. Bacteria including Ps-4 generally recovered and grew in PBW at greater rates than uniodinated controls.
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