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In the Closed Ecology Experiment Facilities (CEEF), with integrating the Closed Plantation Experiment Facilities (CPEF) and the Closed Animal Breading & Habitation Facilities (CABHF), closed habitation experiments without material exchange with the outside will be conducted after the 2005 fiscal year. Cultivation experiments of about 30 crops and the integrating test of the material circulation system required for the closed habitation experiments have been performed since 2000 fiscal year. Using data reported in these experiments, material circulation in CEEF is simulated based on the recent operation strategy, and the storage capacity needed for the buffer of an air processing subsystem was estimated. In order for two humans to dwell over 120 days, the storage capacities of the carbon dioxide tank, the oxygen tank, and the waste gas tank in CPEF, and the carbon dioxide tank and the oxygen tank in CABHF are 820 g, 2830 g, 4425 g, 1780 g, and 1792 g, respectively.

Simulation of material circulation for a closed experiment using CEEF consisting of a plant and human system was performed. Initial set of materials contained in CEEF was decided by a decision procedure. Before the closure where the plant system is operated independently, the plant system (CPEF) needs gas exchange of O2 and CO2 with the outside. After the closure where the plant and human system are operated in a cooperation mode with mutual material exchange, no exchange of materials is needed. The closure time corresponds to the longest cultivation period to be cultivated in CPEF.