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A detailed study was conducted on nitrification using a bench top bioprocessor system proposed for water recycling of a urine-soap wastewater expected to be generated by crewmembers on International Space Station (ISS) or similar long-term space missions. The bioprocessor system consisted of two packed bed biofilm reactors; one anoxic reactor used for denitrification and one aerobic reactor used for nitrification. lnfluent wastewater was a mixture of dilute NASA whole body soap (2,300 mg/L) and urea (500 mg/L as organic nitrogen). During two months of steady-state operation, average chemical oxygen demand (COD) removal was greater than 95%, and average total nitrogen removal was 70%. We observed that high levels of nitrite consistently accumulated in the aerobic (nitrifying) reactor effluent, indicating incomplete nitrification as the typical end product of the reaction would be nitrate.

Water reuse is essential for long duration space missions. However, water recycle systems also provide a habitat for microorganisms and allow accumulation of chemical compounds which may be acutely or chronically toxic to mission crew members. Contaminant fate and accumulation in closed-loop water recycle systems is being investigated at the University of Colorado and Martin Marietta as part of the activities of the Center for Space Environmental Health (CSEH), a NASA Specialized Center of Research and Training (NSCORT). The water contaminant distribution research uses a scaled-down physical model of a water (shower, laundry, urine and/or condensate) recycle system to analyze for and model four “indicator” contaminants: viruses and bacteria, nitrogen species, and selected organic and inorganic compounds. The water recycle test bed is comprised of five or more individual water treatment processes linked in a closed loop, and spiked with chemical and biological contaminants.

Until now, NASA's space water reuse research program has not considered the transport of water borne infectious enteric viruses; however, viral diseases probably are a significant concern in long duration space missions. To simplify monitoring and prediction of pathogen distribution, model indicator strains historically have been used. In this research, the male specific RNA coliphage MS-2 is used as a model of enteric viruses due to their similar size and biochemical composition. Inactivation of some water borne enteric viruses by iodine has previously been characterized. In this paper, iodine inactivation of the model coliphage MS-2 in buffered water is compared with earlier bench-scale disinfection survival data and with survival in iodinated simulated shower water used in a test water recycling system.

Iodine is being considered for disinfection of recycled hygiene and potable water in Space Station Freedom. Like chlorine, the halogen iodine can form disinfection by-products (DBPs) when used as a disinfectant in waters with dissolved or colloidal organic compounds. Recycled shower and laundry wastewater, urine and condensate from the space cabin atmosphere all have large amounts of dissolved and colloidal organic compounds and may generate iodinated DBP's which can be toxic to humans. We have investigated the formation of iodinated DBP's (IDP's) in model compounds typical of shower wastewater and condensate. The selection of these model compounds and flask experiments to test for IDP formation have been described. Methods for reaction, extraction and analysis for IDP's also have been developed. We have tentatively identified likely organic precursors from recycled water and several iodinated organic compounds formed during the reaction with iodine.

Astronauts working in space for extended periods of time will be in a closed environment where both air and water are recycled. As the lengths of missions increase, the astronauts are likely to be at greater risk for viral infection by consumption of recycled water and air. In order to minimize the risk of infection and assure mission success, indicators of the viral quality of recycled water and air will have to be determined. The indicators chosen will depend upon many factors arising from the fields of engineering, public health, virology, and infectious disease. Moreover, selection of viral indicators must address the unavoidable mixing of recycled air and water and the pathogens they contain. This paper is a discussion defining viral quality indicators for potable recycled water and air that reflect the behavior of viruses in a closed, multimedia, space environment The applicability of indicators currently used on earth are considered for use in space.

The Biological Wastewater Processor Experiment Definition team is performing the preparatory ground research required to define and design a mature space flight experiment. One of the major outcomes from this work will be a unit-gravity prototype design of the infrastructure required to support scientific investigations related to microgravity wastewater bioprocessing. It is envisioned that this infrastructure will accommodate the testing of multiple bioprocessor design concepts in parallel as supplied by NASA, small business innovative research (SBIR), academia, and industry. In addition, a systematic design process to identify how and what to include in the space flight experiment was used.