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Biodiesel is an alternate fuel for diesel consisting of the alkyl monoester of fatty acids derived from vegetable oils. The most usual method to transform oil into biodiesel is transesterification which can be carried out using different catalyst. Jatropha is second generation oil which is non edible and can be use for producing biodiesel. The first part is to expel oil from jatropha seeds. There are different types of expelling methods such as mechanical extraction, solvent extraction and enzymatic extraction. The study was conducted with hand driven mechanical expeller which is most conventional way of extracting oil from seeds with mechanical efficiency of 60-80% for single pass. The study includes various combinations of parameters like seed treatment, sun drying, pre-heating, soaking at different temperatures and different de-hulling compositions.