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A 48-hour manned closed environment test was carried out using the closure environmental adaptation training facility (isolation chamber) at Tsukuba Space Center of NASDA (National Space Development Agency of Japan), in Tsukuba-shi, Ibaraki, Japan. This 48-hour closed test was planned and conducted as a preliminary testing for future manned closed tests of advanced life support systems. The purposes of the test were to obtain basic data related to design requirements of regenerative life support systems, which include environmental data of air, water, waste water, and microorganisms inside the chamber, and data on human mass balance and human consumables during the test. Additionally perform integrated evaluation tests on functions and performance of Environmental Control and Life Support System (ECLSS) subsystem research models which have been fabricated and evaluated so far in independent separate tests.