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The efficiency and output of commercial agricultural as it exists today is greatly influenced by natural environmental conditions. Production under a controlled environment such as a Vegetable Factory concept may be considered to overcome these problems. The Modular Growing Component Salad Machine (MGCSM), which was built in 1992, has recently undergone major design changes. Use of red and blue LEDs are being considered to replace high-pressure sodium (HPS) lamps. This modification can reduce mass, weight and energy consumption. If this growing system can be manufactured at an affordable cost to be used for profitable agricultural production on Earth for growing flavorful, pesticide free, healthier vegetables with minimal labor requirements.

We have reported that Euglena, a photosynthetic alga, has the ability to grow in a high CO2 atmosphere and yield great biomass due to the induction of chlorophyll biosynthesis under high CO2 conditions. In the present study, we investigated some microalgal culturing systems for CO2 elimination and O2 regeneration in closed air on the ground by using the photosynthetic alga, Euglena gracilis. z. In the first part of our experiments, we focused on the effects of CO2 and light intensity in the present culture system. We investigated to determine the optimum culture conditions for a microalgal culture system that functions effectively in the controlled ecological life support systems (CELSS). We determined the maximum elimination rate under a high concentration of CO2 with stirring of the supply gas by bubbling. We found that the maximum CO2 elimination rate or gas exchange performance under a 10 % concentration of CO2 was about 2.3 times higher than its low concentration of CO2 (0.04 %).