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The piloted Mars sprint scenario of NASA's “Humans to Mars” initiative involves round-trip “sprints” with a 2-week exploration of the Martian surface. This paper investigates the bio-isolation dynamics of plants and humans in a piloted Mars sprint. To simulate a life support system for a crew of six, a transient, thermal-network model is used. Two crops, lettuce and winged beans, are chosen for a cabin greenhouse. The crew cabin and the greenhouse are physically separated but dynamically interfaced with mass and energy flows. The plants provide the bio-regenerative portion of air, water, food, and waste cycles. The percentage of contribution by bio-regeneration to air revitalization, water reclamation, wet food supply, and waste processing functions are 9, 29, 22, and 50 percent, respectively.