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Over the past decade, society’s concern for the state of the environment has increased. Several actions have been taken by governments, non-governmental organizations, public and private environmental and health agencies to limit pollutant emissions. Within this context, the control of vehicle emissions has become increasingly stringent. Emission limits have been substantially reduced, and the role of unregulated emissions is increasing in importance. Ammonia is the third most abundant nitrogen compound in the atmosphere. Ammonia emissions have received special attention due to their contribution to secondary particulate matter production in the forms of ammonium nitrate (NH4NO3) and ammonium sulfate ((NH4)2SO4). The aim of this work was to evaluate the effect of fuel sulfur content on ammonia emissions in a Euro III motorcycle.