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The influence of ethanol blending in Diesel fuel on the spray and spray combustion characteristics was investigated by performing experiments in an optically accessible high-pressure / high-temperature spray chamber under non-evaporating, evaporating and combusting conditions. Three fuels were investigated: (1) Diesel - a European Diesel based on the EN590 standard; (2) E10 - a blend of Diesel containing 10% ethanol and 2% emulsion additive; and (3) E20 - a blend of Diesel containing 20% ethanol and 2% emulsion additive. A constant gas density of 24.3 kg/m3 was maintained under non-evaporating (30 °C, 21.1 bar), evaporating (350 °C, 43.4 bar), low combustion temperature (550 °C, 57.3 bar) and high combustion temperature (600 °C, 60 bar) conditions. A single-hole injector with a nozzle diameter of 0.14 mm was used and injection pressure was held constant at 1350 bar. Various optical methods were used to characterize the non-combusting and combusting sprays.