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The objective of this program, which is supported by the U.S. Department of Energy (DOE) through the National Renewable Energy Laboratory (NREL), is to provide an unbiased and comprehensive comparison of transit buses operating on alternative fuels and diesel fuel. The information for this comparison was collected from eight transit bus sites. The fuels studied are natural gas (CNG and LNG), alcohol (methanol and ethanol), biodiesel (20 percent blend), propane (only projected capital costs; no sites with heavy-duty propane engines were available for studying operating experience), and diesel. Data was collected on operations, maintenance, bus equipment configurations, emissions, bus duty cycle, and safety incidents. Representative and actual capital costs were collected for alternative fuels and were used as estimates for conversion costs. This paper presents preliminary results.