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A biological treatment process employing immobilized microbial populations was field tested on contaminated ground waters and industry effluent having elevated concentrations of volatile organics, semi-volatile organics and organic pesticides, respectively. The process, consisting of a packed bed biological reactor, containing specific adapted microbial strains immobilized on a porous diatomaceous earth support was operated in a plug flow configuration over an extended period. General process measurements included temperature, pH, total organic carbon and COD. Microbial adenosine 5′-triphosphate (ATP) measurements provided estimates on immobilized biomass performance. Specific chemical analyses of waste stream constituents were determined using gas chromatography/mass spectroscopy (GC/MS) methods for both feed and treated streams.

A biological treatment process employing immobilized microbial populations was field tested on contaminated ground water having elevated concentrations of volatile organics, primarily ethylene dichloride (EDC), 1, 1 dichloroethane and 1,1,2 trichoroethane. The process, consisting of a 75 L packed bed reactor containing specific adapted microbial strains immobilized on a porous diatomaceous earth support, was operated in a plug flow configuration over a 14 day period in a semi-continuous mode, i.e., draw and fill. General process measurements included temperature, pH, and titrated chlorides. Microbial adenosine triphoshate (ATP) measurements provided estimates on immobilized biomass performance.