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It has been widely reported that the overall vibration comfort performance of static and dynamics seats is strongly influenced by the biodynamic behaviour of the seated human body. The contributions of the seated occupant to the overall vibration attenuation of the coupled seat-occupant system are experimentally investigated as functions of the nature of excitation, static and dynamic properties of the seat, and the sitting posture. The study involved two different seats with natural frequencies in the vicinity of 1.5 Hz and 4 Hz, which would characterize the low natural frequency suspension as well as high natural frequency seats employed in automobiles and some industrial vehicles. The vibration isolation properties of the seats are evaluated with a rigid mass and two human subjects under different vibration excitations, including swept sine, broad-band random and standardized vibration spectra of selected vehicles.