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In this study exhaust emissions from a commercially available motor vehicle with neat diesel fuel and diesel/biodiesel blends have been investigated. The vehicle was a EURO IV class with a turbocharged direct injection engine (TDI) of 2.0 liter displacement. Methyl ester obtained from used frying oil was as the blendstock at proportions of 5, 20, and 50 % by volume. On-board emission measurements were conducted in real-world driving on a specific driving route infrastructure including altitude differentiation, humps, and various alternations on driving conditions, such as frequent accelerations and decelerations. The addition of biodiesel demonstrated a decrease in NOx, CO and smoke opacity emissions. CO2 emissions and fuel consumption showed an increase