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In the last few decades a significant effort has been stablished in the automotive industry as well as in academic community towards increasing the renewable fuels applications in internal combustion engines, such as alcohol and gas derived sources. Meanwhile, turbo charging direct-injection spark-ignition engines have become fundamental features to achieve downsizing purposes, increasing power generation efficiency and attending high restrictive emissions regulations that have being taking place recently. For this study, experimental tests were carried out in a single cylinder research engine considering direct injection (DI) and port fuel injection (PFI) operations with anhydrous ethanol. The aim of this paper is to present a review and conduct further investigation about methodologies applied for imaging post processing considering chemiluminescence technique applied in an optical research engine.