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Drivers' fatigue during long hour driving comes from various causes. The purpose of this study is to clarify the mechanisms of drivers' fatigue in relation to seat performance. The drivers' posture was examined on the assumption that an optimal seating posture against fatigue should exist. As a first step, laboratory test was conducted to grasp fatigue analysis viewpoints. Then, as a next step, road test with seat pressure distribution (S.P.D.) and Electromyogram (EMG) was implemented to clarify fatigue process. Results show that seat support condition is found to be one of the factors behind uncomfortable postures and physical burdens, which leads to subjectively felt fatigues.