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Nowadays, many researches are being carried out to replace the diesel by alternative fuels. Biodiesel and ethanol are strong candidates for this purpose. However, the experimental study of the combustion of biofuels in engines is not an easy task. Due to the large differences between the properties of the new fuels and the conventional diesel, radical changes may be needed in current engines, developed specifically for the fossil fuel. So, the experimental study of ethanol compression ignition (CI) combustion is not simple to be obtained in conventional engines. Therefore, some experimental apparatus, such as a rapid compression machine (RCM), are useful to conduct this kind of study. This paper describes the RCM adaptations made in order to run CI combustion tests using Ethanol-Powered (ED95) and Diesel (S50) for different compression ratios and injection timing.