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Planar laser-induced fluorescence of 3-pentanone doped into the fuel (iso-octane) and OH, which is present in the combustion products, was performed in an optically accessible direct-injection spark-ignition (DISI) engine under stratified and homogeneous operating conditions. A wall-guided, swirl-based combustion chamber was utilized, and experiments were performed for light load, where the fuel-air equivalence ratio was 0.3, and high load conditions, with an equivalence ratio of 0.7, at speeds of 600 and 1200 rpm. The 3-pentanone images were calibrated through the use of a premixed charge condition of known equivalence ratio, with corrections applied for number density changes due to combustion. At the light load condition combustion of the highly stratified fuel cloud was directly measured for the first time. The equivalence ratio of the mixture at the flame front was found to be in the range from 0.5 - 0.8 for optimized combustion conditions in this engine.