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A comparison between conventional and bio-derived fuel spray characteristics is described in this paper. Radial distributions of drop size, axial and radial velocity components were measured using a Phase-Doppler Anemometer (PDA). A digital visualization system including a CCD camera was used to estimate the spray tip penetration. Fuel was injected through a single hole diesel injector into a test section at ambient pressure. An empirical expression was found to estimate the spray tip penetration at ambient conditions.