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In the course of CRYOSYSTEM phase B (development phase) financed by the European Space Agency, AIR LIQUIDE (France) and Astrium Space Infrastructure (Germany) have developed an optimized design of a −183°C freezer to be used on board the International Space Station for the freezing and storage of biological samples. The CRYOSYSTEM facility consists of the following main elements: - the CRYORACK, an outfitted standard payload rack (ISPR) accommodating up to three identical Vial Freezers - the Vial Freezer, a dewar vessel capable of fast and ultra-rapid freezing, and storing up to approximately 900 vials below −183°C; the dewar is cooled by a Stirling machine producing > 6 W at 90 K. The Vial Freezer is operational while accommodated in the CRYORACK or attached to the Life Science Glovebox (LSG). One CRYORACK will remain permanently on-orbit for several years while four Vial Freezers and two additional CRYORACKs support the cyclic upload/download of samples.