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Natural gas has a favorable effect on both the engine performance and emissions. The heavy duty vehicles fueled with natural gas have very low emission levels, not easily obtainable with other fuels, and therefore they are considered the most practicable EEV (Enhanced Environmental Friendly Vehicles). Moreover, the assessment of fuel availability makes its use possible for a long time, whilst the new generation of feeding systems offers solutions to the problem of fuel composition variability. Nevertheless, the positive environmental aspects often are not totally known by people involved in natural gas application, therefore some limitations in the diffusion of this fuel in the transportation sector still appear. This paper refers about the activities, under development at the Istituto Motori, aimed to evaluate the toxicity of engine exhausts gas, based on the analysis of components harmful to human health, such as the carbonyl compounds, PAHs and particulate matter.