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Bacillus sp. ATCC 29669 was isolated from microbial fallout in clean rooms during the assembly of the Viking Spacecraft missions to Mars, making it a potential contamination concern for outbound space missions. Spores from this bacterial strain were found to be thirty times more resistant to dry heat than B. atrophaeus. Spore inactivation rates under vacuum controlled humidity were faster than rates obtained under ambient humidity. Inactivation rates for these heat resistant spores are important considerations for planetary protection implementation where temperature, time and humidity conditions are used to estimate the effectiveness of dry heat microbial reduction (DHMR) procedures.

This study of samples collected from Mars 01 Orbiter was conducted to gain a better understanding and practical experience in methods to process and preserve samples intended for planetary protection analysis. Samples were evaluated for the viable growth of microbes, the molecular biomarker adenosine triphosphate (ATP), and the presence of lipopolysaccharide, a bacterial cell wall component. Losses were observed in the number of viable microbes after freezing as well as in the detectable lipopolysaccharide. Two independent studies of pooled cleanroom samples demonstrate good ATP recovery and consistent values after freezing at −20 °C.