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In the closed environments, removal of trace contaminants generated from persons, animals, and plants is important function to keep the environment below the allowable level. We conducted the fundamental tests in order to confirm design of TCCA (Trace Contaminants Control Assembly) for Closed Ecology Experiment Facilities (CEEF), and obtained the following results; 1) The palladium-on-alumina catalyst is suitable for CO, CH4, C2H4 conversion at temperature lower than 400°C. 2) The alkali impregnated AC (activated charcoal) is effective for NO2, SO2 removal and prevents catalyst poisoning from SO2. 3) The active-desorbing conducted by hot air blow-throw an AC is effective for C2H5OH, CH2Cl2 desorbing. We discuss the fundamental test and design conditions for TCCA.