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Planar Laser-Induced Fluorescence has been widely accepted and applied to measurements of fuel concentration distributions in IC engines. The need for such measurements has increased with the introduction of Direct Injection (DI) gasoline engines, where it is critical to understand the influence of mixture inhomogeneity on ignition and subsequent combustion, and in particular the implications for cyclic variability. The apparent simplicity of PLIF has led to misunderstanding of the technique when applied to quantitative measurements of fuel distributions. This paper presents a series of engineering methods for optimizing, calibrating and referencing, which together demonstrate a quantitative measure of fuel concentration with an absolute accuracy of 10%. PLIF is widely used with single component fuels as carriers for the fluorescent tracers.