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In this study the influence of various blends biodiesel on steady state exhaust emissions was determined. A series tests were conducted over a period of six months, including the summer when the ambient temperature is quite high in Greece. An old technology Ford Escort 1.6L, 4 cylinders with indirect injection system was used on a chassis dynamometer. The test car was not equipped with an engine Electronic Control Unit (ECU) and run on the dynamometer with full load on three different gear settings (second gear, third gear and fourth gear). Seven fuels were used, a high sulfur diesel, and blends of 10%, 20%, 30%, 40% and 50% by weight biodiesel in neat diesel (B10), (B20), (B30), (B40), (B50) and (B100) respectively. Fuel injection timings were held the same for the biodiesel blends and the baseline diesel fuel to eliminate the potential injection timing differences due to the different fuel heating values.