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Biobutanol, i.e. n-butanol, as a second generation bio-derived alternative fuel of internal combustion engines, can facilitate the energy diversification in transportation and reduce carbon dioxide (CO2) emissions from engines and vehicles. However, the majority of research was conducted on spark-ignition engines fuelled with n-butanol and its blend with gasoline. A few investigations were focused on the combustion and exhaust emission characteristics of homogeneous charge compression ignition (HCCI) engines fuelled with n-butanol-gasoline blends. In this study, experiments were conducted in a single cylinder four stroke port fuel injection HCCI engine with fully variable valve lift and timing mechanisms on both the intake and exhaust valves. HCCI combustion was achieved by employing the negative valve overlap (NVO) strategy while being fueled with gasoline (Bu0), n-butanol (Bu100) and their blends containing 30% n-butanol by volume (Bu30).