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The objective of the project was to compare the exhaust gas emissions and fuel consumption of a test vehicle, which was on a one hand operating with premium gasoline 95 RON (RON 95) and a mixture of 90% by volume premium gasoline 95 RON and 10% by volume of high purity bio ethanol (E10) on the other hand. As a test vehicle a Skoda Octavia station wagon was used. The engine of the tested vehicle corresponded to the Euro 4 emission standard. The investigations were conducted under the real world conditions, and also at the chassis dynamometer test bench. The tested vehicle was equipped with a new On-board measurement System (OBM) to determine the mass emissions on real world driving routes. The measurement method is based on modal analysis of the emission concentrations in the tailpipe of the vehicle, and real time exhaust mass flow determination.