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Optical motion capture (OMC) is a relatively new experimental tool used in many branches of science and engineering. Despite OMC’s widespread use, literature and practical procedures on the quantification of error and uncertainty in OMC systems for rigid bodies are currently underdeveloped. However, in most studies involving error and uncertainty quantification, the OMC volumes are relatively small (maximum length of 2m in any dimension) and involve expensive experimental apparatuses. Therefore, a cost-effective procedure to quantify the positional errors and uncertainties present in a large volume OMC system is presented. The procedure utilizes the kinematics of a wooden block traveling through air to predict errors and uncertainties in the OMC system by only collecting trajectory data.