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The distribution of fuel-air mixture inside the engine cylinder strongly influences the combustion process. Planar laser-induced fluorescence (PLIF) is commonly used for fuel distribution measurement, however, it is mostly reported on moderate- to large-sized engines. In the present work, PLIF is applied to measure the fuel distribution inside the cylinder of a small, four-stroke, port-fuel-injection (PFI), spark-ignition engine with displacement volume of 110 cm3. Iso-octane was used as the base fuel, and 3-pentanone (15% by volume) was added as a fluorescent tracer in the base fuel. The effect of equivalence ratio, considering ϕ = 1.2, 1.0, and 0.8, on in-cylinder fuel distribution was studied with low throttle opening of 25% at 1200 rpm. PLIF images were recorded at different crank angle degrees during both intake and compression strokes over a swirl measurement plane located at the TDC position.