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The heat pump with low global warming potential (GWP) refrigerants is imperative for the electric vehicle (EV) to slow down global warming and extend the driving range while meeting passengers' thermal comfort in low ambient temperatures. However, there are no appropriate refrigerants. To provide long-term and environmental-friendly refrigerants in the heat pump for EVs, herein, we reported newly developed low-GWP refrigerant mixtures, i.e., DL3B, whose GWP is lower than 140, the flammability (lower flammability limit and burning velocity), saturation pressure, lubricant miscibility, material compatibility were experimentally tested. A test bench that can investigate the performance of an R410A prototype was built. The drop-in tests of the DL refrigerant were carried out to evaluate the capacities and COPs for both cooling and heating modes in the EV heat pump system.