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The formation of NO was investigated in a spray-guided spark-ignition direct-injection gasoline engine. The influence of variations in intake air temperature and simulated exhaust gas recirculation was examined in an optical single-cylinder engine, fueled with iso-octane. Cycle-resolved simultaneous measurements of OH-chemiluminescence, NO laser induced fluorescence, and fast NO exhaust gas sampling allowed a detailed view of the formation process of NO in this engine. Overall, it was found that cycle-resolved information is needed to explain the differences found between operating conditions, since the initial high stratification of fuel leads to large spatial gradients in the NO concentration. Averaged in-cylinder NO distributions do not adequately reflect the formation process rather than show a smoothed distribution that may even be counter-intuitive based on averaged chemiluminescence data.