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The formation of particles in the combustion chamber of a direct injection diesel engine has been studied with the use of the Total Cylinder Sampling Method. With this method, nearly the entire contents of the cylinder of an operating diesel engine can be quickly removed at various times during the combustion process. The particle mass and size distributions present in the sample can then be analyzed. If quenching of the combustion process is quick and complete, the resulting samples are representative of the particle mass and size distributions present in the cylinder near the time sampling begins. This paper discusses the effect of injection timing and piston bowl shape on the particle formation and oxidation. Example size distribution measurements are also shown. The particle concentrations in the cylinder were measured for three different injection timings with the standard piston installed in the engine.